Collaborating with the University of Oxford, Professor Ho Yin Edwin Chan’s research team from the School of Life Sciences of The Chinese University of Hong Kong (CUHK) recently unveiled the counteracting relationship between pre-mRNA-processing factor 19 (Prpf19) and exocyst complex component 7 (Exoc7) in controlling the degradation of disease protein and neurodegeneration of the rare hereditary ataxia. The research findings have been published in the prestigious scientific journal, Cell Death & Disease.
Protein misfolding contributes to the pathogenesis of SCA3
Proteins play a significant role in every single cell development in the human body, including neurons. Numerous studies have proved that misfolds and aggregation of proteins contribute to many occurrences of human diseases. Proteins need to adopt proper folding and architecture before being able to execute their biological functions. Even a minor improper assembly of a protein may result in cellular malfunctioning, leading to toxic insoluble protein aggregates that cause diseases. Progressive misfolding of proteins and aggregates will interfere with the functionalities of other normal proteins, and these are also detected in the deteriorating neurons of SCA3 and other protein misfolding induced disorders, including polyglutamine (polyQ) diseases.
Prpf19 is capable of degrading toxic expanded SCA3-polyQ protein
Professor Edwin Chan, Postdoctoral Fellow Dr. Zhefan Stephen Chen, and the team discovered that the nuclear-localized protein Prpf19 is responsible for scrutinizing the quality of SCA3-polyQ, the disease protein of SCA3 or MJD. Potentiating the function of Prpf19 promotes degradation of faculty SCA3-polyQ protein via a process called ubiquitin-proteasome degradation. In this, the toxicity of SCA3 cells is proved to be alleviated, and improvement is also shown in the condition of neurodegeneration and the nervous system of the animal model with SCA3 disease.
The rivalry between Prpf19 and Exoc7 in controlling neurodegeneration of SCA3
Exoc7 is a protein that controls trafficking of proteins within cells, and it is also known as an associating partner of Prpf19. While the coiled-coil domain of Exoc7, a special region of the protein, is crucial for Exoc7 to restrain Prpf19 from functioning, the research team has further discovered that Exoc7 will shuttle to the cell nucleus where it binds directly to Prpf19 and interferes with the pre-mRNA splicing, causing Prpf19 to lose its beneficial effects on SCA3 or MJD disease models.
Professor Chan said, “The current study demonstrates an intricate relationship between Prpf19 and Exoc7, two crucial proteins in nerve cells. Elucidating the mechanism of action of protein networks that govern protein aggregation will allow us to develop potential small molecules or activators targeting Prpf19, with the hope of providing novel strategies for curing SCA3/MJD and other neurodegenerative disorders. Today (28 February) marks the Rare Disease Day 2021. SCA3/MJD belongs to the category of rare neurodegenerative diseases, I also hope our findings will provide an insight into rare disease translational biomedicine research.”